| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180227 |
| Chromosome: | chromosome 12 |
| Location: | 6143539 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536050 | ALA1 | (1 of 3) K14802 - phospholipid-transporting ATPase [EC:3.6.3.1] (DRS2, ATP8A); Phospholipid-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCGTGTGCTGCGGATAGCCGAAGAGGC |
| Internal bar code: | CTTAAGGGGGGAATATTATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 97.1548 |
| LEAP-Seq n confirming: | 1161 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGATGAACAGGATGGG |
| Suggested primer 2: | TGCGCTTGTAGCTGTATTGC |