| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180227 |
| Chromosome: | chromosome_12 |
| Location: | 6143540 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g536050 | ALA1 | Phospholipid-transporting ATPase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCGTCCTCGGCGCGGGTGGAGGAGCTGGA |
| Internal bar code: | AGTATGGGCTGTCATGTTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 252 |
| LEAP-Seq percent confirming: | 88.5952 |
| LEAP-Seq n confirming: | 3721 |
| LEAP-Seq n nonconfirming: | 479 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGATGAACAGGATGGG |
| Suggested primer 2: | TGCGCTTGTAGCTGTATTGC |