| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180256 |
| Chromosome: | chromosome_10 |
| Location: | 3062838 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g441500 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGAAGAAGTCTTCCCGGCGCTGCACCTCA |
| Internal bar code: | CGTGATCTTGCGGTTCGAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 99.84 |
| LEAP-Seq n confirming: | 4368 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACACCATCACTTCCAC |
| Suggested primer 2: | GGGACGCATTATCACAGCTT |