| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180262 |
| Chromosome: | chromosome 5 |
| Location: | 3359168 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240750 | TEF17 | Predicted protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACTGGAACCGCAACCGCCGAGAAAGCC |
| Internal bar code: | TGACGCACTCTCGCGGTGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 98.6532 |
| LEAP-Seq n confirming: | 293 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGATCCGAGCATGTACGA |
| Suggested primer 2: | ACACTCAACCGTGGGAAGAC |