| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180263 |
| Chromosome: | chromosome 6 |
| Location: | 6420841 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292350 | AOC4 | (1 of 1) PTHR11785//PTHR11785:SF355 - AMINO ACID TRANSPORTER // SUBFAMILY NOT NAMED; Amino acid carrier | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGCTGCTCCTCGTATGTATCCTCACGC |
| Internal bar code: | CCAACAGGCACGTAACTTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1038 |
| LEAP-Seq percent confirming: | 99.3721 |
| LEAP-Seq n confirming: | 3007 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGCTCCACTTGGTAGGG |
| Suggested primer 2: | ATAGGCACACAGGAAGGTGC |