| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180319 |
| Chromosome: | chromosome 10 |
| Location: | 6498650 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466500 | CPL12 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAACTGCCTGGAGTGCGTGGAGATGGAG |
| Internal bar code: | AATAAGAGCTATCTGGTCACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 837 |
| LEAP-Seq percent confirming: | 99.7483 |
| LEAP-Seq n confirming: | 4359 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTCAGTTTGCATTTGA |
| Suggested primer 2: | TACTGCTGCATAAACGCCTG |