Insertion junction: LMJ.RY0402.180375_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.180375
Chromosome:chromosome 17
Location:1069580
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g703750 (1 of 1) PF14222 - Cell morphogenesis N-terminal (MOR2-PAG1_N) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGCGGCGAGCTGTCGGTGGATCCGGAGG
Internal bar code:CATTTAAAGTGCAGCTTTTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:333
LEAP-Seq percent confirming:96.0934
LEAP-Seq n confirming:6666
LEAP-Seq n nonconfirming:271
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTAAGCTCCCCACCACACAT
Suggested primer 2:GCGGATCCTACCACTGATTC