| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180382 |
| Chromosome: | chromosome 17 |
| Location: | 3429880 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGAAGTGTGTACCTTACTTTCCTATCC |
| Internal bar code: | TCGCCGGCGGAAAATCTAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 93.6005 |
| LEAP-Seq n confirming: | 19994 |
| LEAP-Seq n nonconfirming: | 1367 |
| LEAP-Seq n unique pos: | 107 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATATACGGCAGCACGGTCA |
| Suggested primer 2: | CAGACCTTGGGCTAGCTGTC |