| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180391 |
| Chromosome: | chromosome 16 |
| Location: | 3769788 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689300 | STPK24,PTK27,STK24 | Serine/Threonine protein kinase; (1 of 10) PTHR23257//PTHR23257:SF520 - SERINE-THREONINE PROTEIN KINASE // IP11267P | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCGTAGGGTCAGCTGACCCCACAGGGC |
| Internal bar code: | CTAGCCTCTAAGAGGATCGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 99.6016 |
| LEAP-Seq n confirming: | 750 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAAGACGATATCAGCACG |
| Suggested primer 2: | CCTGGATCTGCTGACAACAA |