| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180392 |
| Chromosome: | chromosome 12 |
| Location: | 9677882 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555803 | NDA6 | Type-II NADH dehydrogenase; (1 of 5) 1.6.5.9 - NADH:ubiquinone reductase (non-electrogenic) / Ubiquinone reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCCAAAGGAATGCAGAGAGCTATAGCT |
| Internal bar code: | ACCGCCTGGGAAACTTGCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 90.625 |
| LEAP-Seq n confirming: | 319 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGCATGTGTTCCTTCCT |
| Suggested primer 2: | GTGGGATGTGTTGCTGTTTG |