| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180402 |
| Chromosome: | chromosome 8 |
| Location: | 4733041 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTGGAAGCCAGCCGTAAACCAGCGCAG |
| Internal bar code: | GAGCACCAGCGGAGGGGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 163 |
| LEAP-Seq percent confirming: | 1.99313 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 1426 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCAGCACCTCAAAGAC |
| Suggested primer 2: | GAACAGTGGCAGTGCAAAGA |