| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180417 |
| Chromosome: | chromosome 5 |
| Location: | 645005 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g231050 | (1 of 1) K10636 - autocrine motility factor receptor (AMFR, GP78) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTAAGACCTGAACACTTACAGGCGTGG |
| Internal bar code: | GCGCGCTTGTATAGAAGTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 99.7917 |
| LEAP-Seq n confirming: | 479 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCGAGCCAGTCCTATGG |
| Suggested primer 2: | CCACCTACTTGAAGGACCCA |