| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180423 |
| Chromosome: | chromosome 1 |
| Location: | 5754537 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041100 | HLM3 | (1 of 1) PTHR12197:SF75 - HISTONE-LYSINE N-METHYLTRANSFERASE ATXR2; Histone-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATATGATGCCGACATTCTTTGGGTTGGC |
| Internal bar code: | ACCTATGCGTCGGTATATGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 95.4936 |
| LEAP-Seq n confirming: | 6887 |
| LEAP-Seq n nonconfirming: | 325 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCAAATCCAGATCCA |
| Suggested primer 2: | ATGAACGCGTGGAATAGGTC |