| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180429 |
| Chromosome: | chromosome 12 |
| Location: | 1099747 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490700 | MIN1 | (1 of 1) IPR000008//IPR018392 - C2 domain // LysM domain; Mini-eyespot protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCAAAACATACCCCGCCGCGCACACAGC |
| Internal bar code: | GTTCTGCAGTGCTGACGAAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.199 |
| LEAP-Seq n confirming: | 3839 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGCCCAGGTAAGAGTACA |
| Suggested primer 2: | TGTACTACGAGGTGAGGGGG |