| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180437 |
| Chromosome: | chromosome_3 |
| Location: | 2073874 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g156700 | FAP185 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTTAGTCGGGCCGTGCGTGCCGGCACTCC |
| Internal bar code: | GTAGGGTCGCCGCGGTACGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 28 |
| LEAP-Seq percent confirming: | 88.75 |
| LEAP-Seq n confirming: | 213 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGATTGTGGCTTTTGTT |
| Suggested primer 2: | AATCAAGGGACCCCATTTTC |