Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.180437 |
Chromosome: | chromosome_3 |
Location: | 2073910 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g156700 | FAP185 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCTGACGGCCCTGCAGGATTGCCAGCTGAC |
Internal bar code: | GCCATGTCGACGGCTGAAGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1313 |
LEAP-Seq percent confirming: | 99.596 |
LEAP-Seq n confirming: | 3451 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATCAAGGGACCCCATTTTC |
Suggested primer 2: | CAGGGATTGTGGCTTTTGTT |