| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180440 |
| Chromosome: | chromosome 12 |
| Location: | 63461 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483720 | (1 of 2) PTHR10302//PTHR10302:SF8 - SINGLE-STRANDED DNA-BINDING PROTEIN // PROTEIN OSB2, CHLOROPLASTIC-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACACGCGGGCCCAACCTTGTGCTCAGAC |
| Internal bar code: | CCGGGACTTGGTGCAAGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 99.7621 |
| LEAP-Seq n confirming: | 1258 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAGATGCCAGGTAGGGT |
| Suggested primer 2: | CCTGGTATCCACCTGCTGTT |