| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180446 |
| Chromosome: | chromosome 7 |
| Location: | 4643386 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344350 | TPP1 | Chloroplast thylakoid processing peptidase; (1 of 1) K03100 - signal peptidase I (lepB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTTTCTCCGCAACGGTTTCCCTCAGCT |
| Internal bar code: | CGTCGTGCGGAAGGGGCTTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 997 |
| LEAP-Seq percent confirming: | 99.0109 |
| LEAP-Seq n confirming: | 4705 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCCAGCTGCACTATTCA |
| Suggested primer 2: | CGGAAGAGGTTATTTGCCTG |