Insertion junction: LMJ.RY0402.180458_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.180458
Chromosome:chromosome 9
Location:1695133
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396950 MFT25,CGL15,PHT4I,PHT4-9 Na+-dependent inorganic phosphate cotransporter; (1 of 4) K08193 - MFS transporter, ACS family, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), other (SLC17A) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAAGATATCACCCCGTTGCCCAATGAAC
Internal bar code:GGTAGAACCGGGCGTTGATGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:37
LEAP-Seq percent confirming:90.393
LEAP-Seq n confirming:207
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGACGAAAGTGACGGCTC
Suggested primer 2:ATCCCGCTACAAAATCATCG