| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180494 |
| Chromosome: | chromosome 17 |
| Location: | 6163087 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741700 | KIL22 | Putative kinesin-like motor protein; (1 of 1) PTHR24115//PTHR24115:SF421 - FAMILY NOT NAMED // KINESIN-LIKE PROTEIN KIF25 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCATTGTTAAGTACGGCTCTGGCCCCA |
| Internal bar code: | GACCGCGGGCTCCGGGGTCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTCTTCCTGGGTCTAGC |
| Suggested primer 2: | ACCGCGTATTTCCAACACTC |