Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.180499 |
Chromosome: | chromosome 13 |
Location: | 2401192 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g579582 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACAACGTGCCAACCAAGCCACAAGCTC |
Internal bar code: | GCTTCACGATGAGTACGGCGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 518 |
LEAP-Seq percent confirming: | 70.6579 |
LEAP-Seq n confirming: | 2030 |
LEAP-Seq n nonconfirming: | 843 |
LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCACGGACATCGGTAACAA |
Suggested primer 2: | TGTGTGTGTGTGTTGGATGG |