Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.180512 |
Chromosome: | chromosome 8 |
Location: | 1159811 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g362800 | 5'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTGCTCCAAGTTCCAGACTCGCCCGCG |
Internal bar code: | CTCGTAACGGAACAGCCGAGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 729 |
LEAP-Seq percent confirming: | 99.159 |
LEAP-Seq n confirming: | 1179 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGCTCAAAGTGTCTTTTCC |
Suggested primer 2: | TGACCACGGTTGTAGTTGGA |