| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180519 |
| Chromosome: | chromosome 16 |
| Location: | 5443679 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACGGCATGGAGCGGCATGGATTCAGG |
| Internal bar code: | CGGGCGAAATTCATTCTCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 808 |
| LEAP-Seq percent confirming: | 83.3853 |
| LEAP-Seq n confirming: | 1872 |
| LEAP-Seq n nonconfirming: | 373 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCAGACCGTAGTCAGCG |
| Suggested primer 2: | GAAGAAGAAGGAGGATGGGG |