| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180549 |
| Chromosome: | chromosome 12 |
| Location: | 3608685 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGCAGACGTACGACTCGCACACGGGTA |
| Internal bar code: | CTGTCAGGCCGATCGTTAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 764 |
| LEAP-Seq percent confirming: | 53.2966 |
| LEAP-Seq n confirming: | 4357 |
| LEAP-Seq n nonconfirming: | 3818 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGAGCTTGGCACATATT |
| Suggested primer 2: | GAGGAACTGCTGGTGAGGAG |