Insertion junction: LMJ.RY0402.180556_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.180556
Chromosome:chromosome 9
Location:628006
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g403450 (1 of 6) PF12796//PF13920 - Ankyrin repeats (3 copies) (Ank_2) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTGCTCTCGAACACGCTGCATGCGGCCT
Internal bar code:GTAACGTCTCCCGTGCTCCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:840
LEAP-Seq percent confirming:96.4072
LEAP-Seq n confirming:161
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGTTGTGCCTAAGAGCG
Suggested primer 2:TACGCACTGATGAGTCCAGC