Insertion junction: LMJ.RY0402.180562_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.180562
Chromosome:chromosome 15
Location:1309790
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre15.g641850 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGCGTTGCTACCGACGTCCGTGAAGTCC
Internal bar code:AAGACGCGCGTAAAGGAGAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:525
LEAP-Seq percent confirming:95.5389
LEAP-Seq n confirming:2163
LEAP-Seq n nonconfirming:101
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:AACACAACAAGAATTCCGGC
Suggested primer 2:CATTGACAACAAGGGTCGTG