Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.180565 |
Chromosome: | chromosome_10 |
Location: | 6510909 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g466650 | FAP252 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGACAAGCGCCCAAGAAGATATGGCGCTC |
Internal bar code: | GTTTCGCGTTAGAAGCGTATCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 947 |
LEAP-Seq percent confirming: | 98.5496 |
LEAP-Seq n confirming: | 6115 |
LEAP-Seq n nonconfirming: | 90 |
LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCTACCGTATGGTGCAGTT |
Suggested primer 2: | CAGCACAACTCACTCTCCCA |