Insertion junction: LMJ.RY0402.180565_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.180565
Chromosome:chromosome_10
Location:6510909
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre10.g466650 FAP252 Flagellar Associated Protein sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TTGACAAGCGCCCAAGAAGATATGGCGCTC
Internal bar code:GTTTCGCGTTAGAAGCGTATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:947
LEAP-Seq percent confirming:98.5496
LEAP-Seq n confirming:6115
LEAP-Seq n nonconfirming:90
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTACCGTATGGTGCAGTT
Suggested primer 2:CAGCACAACTCACTCTCCCA