| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180627 |
| Chromosome: | chromosome_2 |
| Location: | 5524471 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g108450 | FAP280 | Flagellar Associated Protein, transcriptional coactivator-like | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCAATCCCGGCTCCAGCATTTCCGGAACCC |
| Internal bar code: | CTTATCCGGCCTAAGTTTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 99.5666 |
| LEAP-Seq n confirming: | 919 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAAGCATGGCTTACCCAC |
| Suggested primer 2: | TCATATGCATGATGCTCCGT |