| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180628 |
| Chromosome: | chromosome 3 |
| Location: | 4274262 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174500 | HDZ1 | (1 of 1) PF00046 - Homeobox domain (Homeobox); Zygote-specific homeobox protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACACCAACGGCACGCATAAACGACCCGA |
| Internal bar code: | GGGGTTTGTTGCTATATGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 99.6656 |
| LEAP-Seq n confirming: | 6855 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGATGACTGGGAGGATGA |
| Suggested primer 2: | TAGCACACGTCAGAAATCCG |