| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180631 |
| Chromosome: | chromosome_12 |
| Location: | 745216 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g495350 | POC12 | Centriole proteome protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCTGCTCCAGCTGCTGCTCCGCCTGTTGT |
| Internal bar code: | GAGTGAAGGTAGATGCTAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 60 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 272 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGATGACAACACGACCAC |
| Suggested primer 2: | AGGTCTTCATTGGGCCTGA |