| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180646 |
| Chromosome: | chromosome 17 |
| Location: | 2960368 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720050 | FTSH2,FHL2 | (1 of 1) PTHR23076:SF72 - ATP-DEPENDENT ZINC METALLOPROTEASE FTSH 2, CHLOROPLASTIC-RELATED; membrane AAA-metalloprotease, chloroplast | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACGACACATCTCCCGCATCAGGGAGCC |
| Internal bar code: | CTGCCGGGTAAAGGATCTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 99.6138 |
| LEAP-Seq n confirming: | 5933 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGGAGAAGGAGACCCTG |
| Suggested primer 2: | ACCAGCTATCACCTTGTGGC |