| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180724 |
| Chromosome: | chromosome 10 |
| Location: | 5439709 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458500 | ASK1,AHD2 | Aspartate kinase; (1 of 1) K00928 - aspartate kinase (lysC) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCTGGTCTTCTCCACGTTGCAGATGAG |
| Internal bar code: | GCGCAGCCACAGTCTAGGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1078 |
| LEAP-Seq percent confirming: | 99.1562 |
| LEAP-Seq n confirming: | 3173 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCACACTTTGTTGTACT |
| Suggested primer 2: | TCACAGCGCTTATGGTGAAG |