Insertion junction: LMJ.RY0402.180729_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.180729
Chromosome:chromosome 2
Location:3329369
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095090 (1 of 25) IPR013216//IPR029063 - Methyltransferase type 11 // S-adenosyl-L-methionine-dependent methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGTCCCATCACCCCTGAGGCTAGCTAA
Internal bar code:CATATAACGGGCTACGCTGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:935
LEAP-Seq percent confirming:97.2704
LEAP-Seq n confirming:3421
LEAP-Seq n nonconfirming:96
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCATTCACTCGCAAGCAC
Suggested primer 2:AGGACGGACTGCAATCAAAC