| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180746 |
| Chromosome: | chromosome 17 |
| Location: | 5087693 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734900 | (1 of 1) 3.4.17.19 - Carboxypeptidase Taq / Carboxypeptidase 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAGAAGGGCTGGGGAGATGGTTGTCTG |
| Internal bar code: | GGCTCCATGTTCTTTCTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 99.4511 |
| LEAP-Seq n confirming: | 1812 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATTTGCACATTACGCCA |
| Suggested primer 2: | TACGACAAGAAGACCGACCC |