Insertion junction: LMJ.RY0402.180748_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.180748
Chromosome:chromosome 17
Location:178754
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g697406 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGTGAAAAACGTGCACCGCACAGGGGAA
Internal bar code:TGGCAGCGCGGAGCACGGCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:561
LEAP-Seq percent confirming:98.9851
LEAP-Seq n confirming:3121
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTCCCGCATACTCCACT
Suggested primer 2:GAGCACTCTTGGGAGGACAG