Insertion junction: LMJ.RY0402.180758_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.180758
Chromosome:chromosome 4
Location:386617
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g217915 NAR1C Formate/nitrite transporter; (1 of 1) IPR000292//IPR023271 - Formate/nitrite transporter // Aquaporin-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCACGCCCCACTCCCGCGGCTTGCTCGC
Internal bar code:CCCGCGAATAGCTCACCTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:195
LEAP-Seq percent confirming:95.8063
LEAP-Seq n confirming:1919
LEAP-Seq n nonconfirming:84
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCAACCACTTCGTTCTCCAT
Suggested primer 2:TCCACGGAGAATATAACCGC