Insertion junction: LMJ.RY0402.180792_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.180792
Chromosome:chromosome 17
Location:3352071
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g723500 (1 of 1) 3.6.3.30 - Fe(3+)-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCAGCCGGGGATGGAGGCTGCGGGGGG
Internal bar code:GCCAAAATCAAGGGCGCGGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:824
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:977
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:AATCCACACCCACATCCACT
Suggested primer 2:GGTGACCACGTTCCAGTTCT