| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180794 |
| Chromosome: | chromosome 1 |
| Location: | 6304490 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044900 | (1 of 5) PTHR10351:SF42 - LD40663P | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACTCTTGAACTGGCAAAACCAATTGCAA |
| Internal bar code: | TAGGTCCTAGGTCCGTGGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 95.9759 |
| LEAP-Seq n confirming: | 477 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGTGTGCTGTGTCGCTC |
| Suggested primer 2: | TCACTGCTGGAAGAGGAGGT |