| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180807 |
| Chromosome: | chromosome 6 |
| Location: | 2710146 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270500 | (1 of 1) PF00759//PF03067 - Glycosyl hydrolase family 9 (Glyco_hydro_9) // Chitin binding domain (Chitin_bind_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACAGGAGGACACACACACACGTCACAC |
| Internal bar code: | GGAAACGCATGAGTGGGTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 419 |
| LEAP-Seq percent confirming: | 98.5901 |
| LEAP-Seq n confirming: | 2937 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTGCTGTTCTCGCCTCC |
| Suggested primer 2: | AAGGCAACAACCATTCAACC |