| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180812 |
| Chromosome: | chromosome 6 |
| Location: | 2714793 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270500 | (1 of 1) PF00759//PF03067 - Glycosyl hydrolase family 9 (Glyco_hydro_9) // Chitin binding domain (Chitin_bind_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCAGCAGTGCTTCTGCCCGGCGGCACG |
| Internal bar code: | GTTGGTGCATTCAACTCCAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1035 |
| LEAP-Seq percent confirming: | 99.391 |
| LEAP-Seq n confirming: | 3427 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCTTCCGTGTCTAGCTT |
| Suggested primer 2: | AATGGTGGAGAGGTCTGGTG |