Insertion junction: LMJ.RY0402.180836_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.180836
Chromosome:chromosome 7
Location:356649
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g314700 (1 of 1) PF14675 - FANCI solenoid 1 (FANCI_S1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGGAACGAGGGACGTCTGTGCCATCGCC
Internal bar code:CCATGGGGGGACATACGAGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:400
LEAP-Seq percent confirming:98.6287
LEAP-Seq n confirming:1870
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AATGTAAACATGCACCGCAA
Suggested primer 2:GAGCCAGTTTGCGAGCTATC