| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180848 |
| Chromosome: | chromosome 10 |
| Location: | 4497026 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451900 | THS1 | Threonine synthase; (1 of 1) 4.2.3.1 - Threonine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGCATCACCGGAGTCGCTGTCCGCACC |
| Internal bar code: | CGGTAGGTGGGAGGGCACCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1003 |
| LEAP-Seq percent confirming: | 98.7584 |
| LEAP-Seq n confirming: | 2068 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTCCTGCCCATCTTCAC |
| Suggested primer 2: | GCTAGCGGCTTAACATTTGC |