Insertion junction: LMJ.RY0402.180866_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.180866
Chromosome:chromosome 9
Location:2791173
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g389000 (1 of 1) PTHR23012:SF61 - AT07234P-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTACCAACTGTTTTAGGTAGTCCCACC
Internal bar code:TGGCTATCGTGCTTTCGATATA

Confirmation - LEAP-Seq

LEAP-Seq distance:550
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:693
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGGATAGGAACCAGGACTGC
Suggested primer 2:GGTTTCGGGTCCCAATCTAT