Insertion junction: LMJ.RY0402.180869_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.180869
Chromosome:chromosome 9
Location:7870125
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g416950 MKP1 (1 of 1) PTHR10159//PTHR10159:SF359 - DUAL SPECIFICITY PROTEIN PHOSPHATASE // SUBFAMILY NOT NAMED; Dual-specificity protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCGGCCCACAGGGTAGCATCCAGGCGA
Internal bar code:GCCATCGCTTGATATGTACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:256
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:341
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAAATACCAAGGGTGCGA
Suggested primer 2:TCCGTGTCTCAAGGAGCTTT