| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180874 |
| Chromosome: | chromosome 7 |
| Location: | 2023817 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325761 | (1 of 2) IPR000104//IPR003439//IPR003593//IPR011527//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC transporter type 1, transmembrane domain // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCGGAGGCTGCGCTGGTATGGGGTTG |
| Internal bar code: | TTGGCTGCGCAATCCTGCCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 99.7623 |
| LEAP-Seq n confirming: | 2518 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGCACTGTCGTTGCCTC |
| Suggested primer 2: | ATCACACGCACCCTTACACA |