Insertion junction: LMJ.RY0402.180874_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.180874
Chromosome:chromosome 7
Location:2023942
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g325761 (1 of 2) IPR000104//IPR003439//IPR003593//IPR011527//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC transporter type 1, transmembrane domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCAACCCACGCACACACTTTTACACACA
Internal bar code:AGACCCAGAAGCTGTCGACTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:171
LEAP-Seq percent confirming:99.4911
LEAP-Seq n confirming:391
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGTAGCACTGTCGTTGCCTC
Suggested primer 2:GAGGTGAGATGAGGCAGAGG