Insertion junction: LMJ.RY0402.180878_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.180878
Chromosome:chromosome 10
Location:3016137
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g441050 (1 of 2) PTHR12321:SF55 - PHD FINGER PROTEIN ALFIN-LIKE 4 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGATTATGAGCAGCAGCTGCTGTGCGCT
Internal bar code:CCAGGGCTGCGGCGCAGTCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:92
LEAP-Seq percent confirming:51.1628
LEAP-Seq n confirming:242
LEAP-Seq n nonconfirming:231
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATTTCGACGGACGGTTACTG
Suggested primer 2:AACAATGCCTCGGAAACAAC