| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180882 |
| Chromosome: | chromosome_9 |
| Location: | 4486311 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g395769 | FAP177 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAACATTGGTGGGTGCCGCGTGGCGGGGT |
| Internal bar code: | CGCGCGTGGTAAATAGAGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 311 |
| LEAP-Seq percent confirming: | 98.2979 |
| LEAP-Seq n confirming: | 693 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGTCCTAGCAGTGGCA |
| Suggested primer 2: | CGATCTCCACCAGGTTCTTG |