| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180887 |
| Chromosome: | chromosome 2 |
| Location: | 657787 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078050 | CAM5 | Calmodulin-like protein; (1 of 1) IPR002048//IPR029530 - EF-hand domain // Cell division control protein 31 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGCGTTGAAAGGGCCCGTGCTGCGCCG |
| Internal bar code: | CACTCATGCCGCCAGAAGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 99.2537 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGTCGGGCATTAAGAT |
| Suggested primer 2: | AGGACTGGGTATGTCGGATG |