Insertion junction: LMJ.RY0402.180887_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.180887
Chromosome:chromosome 11
Location:2191448
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCCTCCGCCGCCTCCGCCTCCACCTCC
Internal bar code:CTATCCCTGAATCCACGGTTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:217
LEAP-Seq percent confirming:96.7533
LEAP-Seq n confirming:1043
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TCCAATGGGTCTCATCACAA
Suggested primer 2:CGCATTACAAGGCAGTCAGA